Frequently Asked Questions

Frequently Asked Questions (FAQs)

This page provides help with the most common questions we get asked.
Have a question not seen under our FAQs? Contact us today.

What are the six reasons for testing under the DOT?

  • Pre-Employment
  • Random
  • Post-Accident
  • Reasonable Suspicion
  • Return-to-Duty
  • Follow-Up

What drugs are tested for in the DOT Drug Testing Program?

The DOT Drug Testing Program tests for five drugs, including:

1.) Marijuana

2.) Cocaine

3.) Amphetamines

  • Amphetamine
  • Methamphetamine
  • Ecstasy

4.) Opioids

  • Morphine
  • Heroin
  • Codeine
  • Oxycodone
  • Oxymorphone
  • Hydrocodone
  • Hydromorphone

5.) Phencyclidine (PCP)

How long does it take to get a test result?

After the laboratory processes the specimen, the results are typically reported to the MRO. Results generally report to the client 24 to 72 hours from the time of collection.

Please note: For DOT testing, the MRO must have a copy of the Custody Control Form (CCF) before the results can be reported to the company.

What methods of confirmation testing for drugs are authorized?

Under DOT regulations, the analytical method must use mass spectrometric identification [e.g., gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), GC/MS/MS, LC/MS/MS] or equivalent.

What do my employees need to take with them to the testing facility?

A form of photo identification such as license, state ID, passport, etc., should be brought to the collection site along with a custody control form (CCF) or an electronic custody control form (ECCF) barcode (often sent to the donor via text or email).  However, the CCF may also be stocked at the collection facility.

What is the custody control form (CCF) or electronic custody control form (ECCF)?

The custody Control Form (CCF) and electronic custody control form (ECCF) are often also referred to as the chain of custody (COC). The chain of custody ensures the specimen belongs to the correct donor. It is the course of action of documenting the management and storage of the specimen from the time the donor gives the sample to the collector, to the lab, and the review and reporting of the final result.

Can an individual add something to his/her urine specimen that would hide their drug use and produce a negative test result?

Many people try to adulterate or substitute their specimen to hide illicit drug use; however, most attempts are revealed in a validity test that the laboratory performs.

What is the minimum number of Follow-Up tests required after a successful Return-to-Duty test?

The Substance Abuse Professional (SAP) may request any number of tests in a follow-up plan; however, there must be a minimum of six tests within the first 12 months of the employee’s return to duty.

Can an employer make arrangements for their employee to a physician if they are unable to provide a sufficient volume of urine during the collection process?

When an employee is unable to produce enough urine for a drug test, the employee must undergo a medical examination to determine if there is a legitimate medical explanation for the “shy bladder.” The employer is required to direct the employee to obtain a medical evaluation from a licensed physician who is acceptable to the MRO. The physician must have expertise in the medical issues raised by the donor’s failure to provide a sufficient specimen.

How long can a donor stay at the collection site to provide a urine specimen?

49 CFR Part 40 requires that a donor be allowed up to three hours after an initial failed attempt to produce a sufficient urine sample. During this time, the donor may drink up to 40 ounces of fluid.